FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823241
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21150.039.4186821
GGTATCA9550.035.3524781
TATCAAC26250.031.621272
TCAACGC26700.031.0883274
CAACGCA27100.030.629465
ATCAACG27300.030.4050673
TCGTATG802.6669059E-630.32118440
AACGCAG28300.029.3289016
GTGGTAT4500.027.5605011
ACGCAGA30600.027.006977
TATACAC2050.026.8817583
CGCAGAG31450.026.2770528
CGTAGAG1208.5952706E-825.84896528
AGAGTAC31650.025.78461611
CAGAGTA31850.025.62270210
GCAGAGT34150.024.098689
TGGTATC4950.023.6573052
TCTCGTA902.0818193E-423.07082738
GAGTACG26850.022.57118812
AGTACGG27050.022.40430313
Produced by FastQC (version 0.11.5)