FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937527
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC12570.13407613860720813No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA11460.12223647958938783No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT10850.11573000030932441No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT9680.1032503597229733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.043.4403421
GTATCAA23700.040.9802361
TCAACGC27300.035.1926774
CAACGCA28050.034.37495
ATCAACG28000.034.312863
AACGCAG28700.033.598176
GTGGTAT2450.032.4472731
TATCAAC29700.032.3488242
ACGCAGA29950.032.194197
CGCAGAG31350.030.7564938
CAGAGTA31300.030.47275510
AGAGTAC31450.030.32741511
TGGTATC2400.030.239752
GCAGAGT32350.029.804169
GTACTAA605.363513E-428.8028341
TTAGTAC605.366863E-428.799763
AGTACGG29200.025.20696413
GAGTACG29200.024.97161312
GTAGTTT5800.024.72628470
ACGGGTA1851.8189894E-1124.28649916
Produced by FastQC (version 0.11.5)