FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937527
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC12870.13727604644986224No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT11000.11732995423065148No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA10720.11434337357750764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.043.5030861
GTATCAA24900.039.8416021
GTAGTTT5750.035.19046470
ATCAACG29350.033.634593
TCAACGC29350.033.5134124
TGGTATC3100.032.310422
CAACGCA30950.032.115435
AACGCAG31400.031.6551786
TATCAAC31050.031.5909792
GTGGTAT3500.030.615961
ACGCAGA32850.030.2579157
CGCAGAG33900.029.4209658
CAGAGTA34250.028.92032810
AGAGTAC34600.028.6293111
GCAGAGT35550.028.3466669
TAAACGT951.04485025E-525.43872620
CGTCTAA851.3664631E-424.54933250
TAATACG1301.8399624E-723.8935724
TATAAGA3650.023.6566622
AGTACGG33500.023.29080813
Produced by FastQC (version 0.11.5)