FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814321
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGATTCAGGGAGA22240.27311097220874814No Hit
CTTCCAACACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC21150.2597255873298122No Hit
GTGTATTACTGCGCGAGATTAGACAGCATAAACTGGTACGCTGGACACTT20600.2529714940422757No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19900.2443753753126838No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19230.236147661671503No Hit
GTGTTGGAAGTGTCCAGCGTACCAGTTTATGCTGTCTAATCTCGCGCAGTAATACACAGCCGTGTCTGCGGCGGT18680.2293935683839665No Hit
GTCTCTGGTGACTCCATTAGTAGTAGTAGTTACTTCTGGGGCTGGATCCG18560.2279199480303222No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17910.21993783778141543No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17450.2142889597591122No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16550.20323680710677977No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16550.20323680710677977No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14780.18150090689052598No Hit
GTCTAATCTCGCGCAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGG14490.17793965770255218No Hit
AGATTAGACAGCATAAACTGGTACGCTGGACACTTCCAACACTGGGGCCA14060.17265918476866002No Hit
CACCTGCACTGTCTCTGGTGACTCCATTAGTAGTAGTAGTTACTTCTGGG13550.16639629826567165No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13480.16553668639271246No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12960.15915099819358705No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12580.15448453374038001No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12040.14785324214898055No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11670.14330957939191058No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11640.14294117430349948No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11560.14195876073440328No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11110.13643268440823705No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10610.1302925996013857No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT10350.127099755501823No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10270.12611734193272678No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10250.12587173854045272No Hit
GTAGTAGGTGTTCCCACTATAATAAATAGTCGCAATCCACTCCAGCTCCTTCCCTGGGGGCTGGCGGATCCAGCC10170.12488932497135649No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGGACACGTCCAAGAACCAGTTCTCCCTGAAT10130.12439811818680839No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA9750.11973165373360137No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9710.11924044694905325No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC9370.11506518928039433No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9350.11481958588812027No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9190.11285475874992786No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC8800.1080654926005838No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8800.1080654926005838No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG8790.10794269090444678No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGGACACGTCCAAGAACCAGTTCTCCCTGAATCTGA8780.10781988920830976No Hit
GTCCCTCACCTGCACTGTCTCTGGTGACTCCATTAGTAGTAGTAGTTACT8720.10708307903148759No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8530.10474984680488408No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGATTCAGGGA8500.104381441716473No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8390.1030306230589657No Hit
CACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGGACA8330.10229381288214352No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC8320.1021710111860065No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8290.10180260609759541No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG8250.10131139931304732No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8240.10118859761691029No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8200.10069739083236218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)