FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862047
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGAAGATACA31200.36192922195657545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25470.2954595283087813No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18510.21472147110308373No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17400.201845143014244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16820.19511697158043587No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16680.1934929301998615No Hit
GGGTACTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16430.1905928563059787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16310.18920082083691495No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16210.1880407912793618No Hit
ACAGTACCCCGTGGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA16200.18792478832360648No Hit
GTGTATTACTGTGCGAGAGATGGTCTCCCCGGTAGTTACCATGCTTTTGA15980.18537272329698962No Hit
GATCTATGCTGCATCCAGTTTTCAAAGTGGTGTCCCATCGAGGGTCAGTGGCAGTGGATCTGGGACAGATTTCA15630.18131261984555366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15240.1767885045710965No Hit
GACTTACAGTACCCCGTGGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT15240.1767885045710965No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15210.17644049570383055No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14920.17307640998692647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14090.1634481646592355No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14030.16275214692470363No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13970.16205612919017176No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13830.1604320878095974No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13740.15938806120779958No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT13470.15625598140240612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13410.15555996366787428No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13360.1549799488890977No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13290.1541679281988105No Hit
CTCCTGTGTAGCCTCTGGATTCACCGTCAATAGCAACTACATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGG13200.1531239015970127No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12830.14883179223406612No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATAATTAGCA12770.14813577449953425No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12620.14639573016320456No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATAATTAGCACTTATTTAAATTGGTATCAGCAGAAA12420.14407567104809832No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12350.14326365035781113No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTC12130.14071158533119424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12040.13966755872939643No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11750.13630347301249235No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGAAGATA11470.1330553902513436No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11320.13131534591501393No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG11180.12969130453443953No Hit
GTACTACAATGTACGCAGGCTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTAT11010.12771925428659922No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10890.12632721881753545No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10450.12122308876430171No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10340.11994705625099328No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10230.11867102373768483No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10160.11785900304739765No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10120.1173949912243764No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10100.11716298531286577No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTCGCTATTTTAAAAGGTGTCCAGTGTGAGATGCAGCTGGTGGAGT9640.1118268493481214No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC9530.11055081683481295No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAAC9310.10799875180819607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9190.10660671633913232No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9080.10533068382582388No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9050.10498267495855794No Hit
GTGTGAGATGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC9020.10463466609129199No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8960.10393864835676013No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8940.10370664244524949No Hit
CTTACAGTACCCCGTGGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAA8810.10219860402043045No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8790.10196659810891981No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8780.1018505951531645No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTCCACGGGGTA8680.10069056559561139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1800.046.0788351
GTATCAA15450.038.2499121
TATCAAC17050.034.4517822
ATCAACG17950.032.7243963
TCAACGC18700.031.411924
GTAGTTT1402.2849545E-630.9237870
CAACGCA19350.030.3567375
AACGCAG19550.030.0461836
AGAGTAC20700.028.03822511
CGCAGAG21100.027.8341568
GTGCTAC756.0903054E-527.6473031
TCTGGAG11900.027.54555170
GCAGAGT21950.026.9136859
ATGGTCA8400.026.50609870
CAGAGTA22450.026.00650210
CAGTTCG10000.025.97597570
TGTATTA8450.024.9436682
ACGCAGA23800.024.67657
GGATTGT851.4279078E-424.3904326
TGTGCGA8550.024.24357810
Produced by FastQC (version 0.11.5)