FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862047
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGAAGATACA27240.31599205147747167No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25620.297199572645111No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20450.23722604451961435No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17350.2012651282354674No Hit
GGGTACTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT17030.19755303365129742No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15740.18258865235886212No Hit
GATCTATGCTGCATCCAGTTTTCAAAGTGGTGTCCCATCGAGGGTCAGTGGCAGTGGATCTGGGACAGATTTCA15640.18142862280130898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15560.18050059915526648No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15020.1742364395444796No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14740.17098835678333082No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14470.1678562769779374No Hit
ACAGTACCCCGTGGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA14450.16762427106642677No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14420.16727626219916084No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14230.1650722060398099No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14020.16263614396894835No Hit
GTGTATTACTGTGCGAGAGATGGTCTCCCCGGTAGTTACCATGCTTTTGA13980.16217213214592707No Hit
GACTTACAGTACCCCGTGGACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT13410.15555996366787428No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13230.15347191046427863No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13160.15265988977399145No Hit
GTACTGTAAGTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12830.14883179223406612No Hit
CTCCTGTGTAGCCTCTGGATTCACCGTCAATAGCAACTACATGGCCTGGGTCCGCCAGGCTCCAGGGAAGGGG12470.14465568582687488No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT12410.14395966809234298No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTC12370.14349565626932173No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12170.1411755971542155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12130.14071158533119424No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12050.13978356168515174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11990.13908754395061984No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATAATTAGCACTTATTTAAATTGGTATCAGCAGAAA11940.1385075291718433No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGATAATTAGCA11790.1367674848355136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11780.1366514818797583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11740.13618747005673704No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11670.13537544936644985No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11430.13259137842832236No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11430.13259137842832236No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10980.12737124541933328No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGAAGATA10590.1228471301448761No Hit
GTACTACAATGTACGCAGGCTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTAT10540.12226711536609952No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10290.11936704147221672No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10240.11878702669344014No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10190.11820701191466358No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10160.11785900304739765No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10120.1173949912243764No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10100.11716298531286577No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10030.11635096462257859No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG9880.11461092028624889No Hit
GTGTGAGATGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGC9650.1119428523038767No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9500.11020280796754701No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9400.10904277840999388No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9360.10857876658697263No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9010.10451866313553669No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8960.10393864835676013No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8920.10347463653373888No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTCGCTATTTTAAAAGGTGTCCAGTGTGAGATGCAGCTGGTGGAGT8890.10312662766647294No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCAGTGATCTGCACTGAAC8820.10231460697618575No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8800.10208260106467514No Hit
GCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTC8780.1018505951531645No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8680.10069056559561139No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8660.10045855968410075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)