FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797600
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.042.8205221
GGTATCA5000.037.331241
TCAACGC16200.035.4172254
ATCAACG16400.034.9831123
CAACGCA16600.034.7720155
TATCAAC16600.034.561632
AACGCAG16900.034.154766
GGCGTTA450.00457886530.725322
AGAGTAC19000.030.19216511
ACGCAGA19450.029.6731647
CAGAGTA19850.028.90111410
CGCAGAG20150.028.8138458
CGTCTAA500.007542925427.74148640
ATCCGCG500.0076617727.65277121
TAGATTG809.438939E-525.9228485
AGTACGG16950.025.28085313
GCAGAGT22950.025.1478449
GAGTACG17200.024.913412
GTATAAG2400.024.4842241
ATAGATT1001.540683E-524.1946564
Produced by FastQC (version 0.11.5)