FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797600
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15900.042.166091
GGTATCA6350.037.552091
TCAACGC18400.036.377614
ATCAACG18400.036.1969033
TATCAAC18800.035.451212
CAACGCA19050.035.136385
AACGCAG19850.033.7203066
ATATCGG450.004608902630.68425823
ACGCAGA21950.030.4942177
AGAGTAC22200.030.15648511
CGCAGAG22800.029.3592118
GTGGTAT2950.029.2871251
CAGAGTA23100.029.1272910
GTGTTAG1551.8189894E-1228.9848061
TGGTATC3100.028.962982
GGCGTAA500.00772372227.6071713
ATAGCGG2300.027.36043263
GCAGAGT24750.027.046069
TAACGGC1555.0931703E-1126.78716536
CGAACCT2500.025.05540853
Produced by FastQC (version 0.11.5)