FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668944
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35750.041.4618071
GGTATCA15400.037.1572151
CAACGCA42100.035.0609325
TCAACGC42350.034.853964
ATCAACG43300.034.3430863
AACGCAG43750.033.8172766
TATCAAC45400.032.775162
ACGCAGA47750.031.2726447
CAGAGTA47850.030.41358610
AGAGTAC48100.030.04363411
CGCAGAG49900.029.7864278
GCAGAGT53650.027.6386459
GTGGTAT5750.026.9774231
CGAACTA4100.026.04947729
AGTACGG45300.025.98090213
GAGTACG46750.025.09996212
CGAGTAT1652.5831448E-422.34759170
TTAACGG4050.022.16022935
TAACGGC3900.022.13676536
GTACGGG52650.021.96176314
Produced by FastQC (version 0.11.5)