FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAACTCCTCAT51290.5264757024637298No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34690.3560819286111676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26080.26770298928161573No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24910.2556933076305617No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23100.23711422746952926No Hit
GTATTACTGTCATCAGAGTGGTAGTTTGCCGTGGACGTTCGGCCAAGGGA22550.23146865062501665No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21580.22151190600833084No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20990.21545574175694457No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20930.21483986064663413No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20480.21022075231930562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19970.20498576288166667No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19820.2034460601058905No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18960.19461843085810715No Hit
GTCCATGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGC18670.19164167215827324No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18320.18804903234812884No Hit
GTGTAAGCTAGTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTC18170.18650932957235267No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18100.1857908016103238No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17790.18260874920705306No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA17410.17870816884175347No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17360.17819493458316138No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG17350.178092287731443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17220.17675787865910367No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16990.1743970010695802No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16810.1725493577386488No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATGACTAC16690.17131759551802786No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16170.16597995922867048No Hit
GAGTGGTAGTTTGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15990.1641323158977391No Hit
ATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA15300.15704968312916873No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15100.15499674609480052No Hit
GTCTCAAGACTTTCAGTCTGTGGCTCCAAAGGAGAGAGTCACCATCACCTGCCGGGCCAGTCAGAACATTGGTA15080.15479145239136372No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14840.15232792795012184No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14650.15037763776747204No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14200.14575852944014356No Hit
GTACTATGTGGGCTCTGTGAAGGGCCGACTCACCATCTCCAGAGACAACG14040.14411617981264896No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13550.13908648407844681No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTGGTAGTTTG13550.13908648407844681No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13490.13847060296813635No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG13240.1359044316751761No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13130.13477531630627357No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12720.13056679538581872No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC12660.12995091427550826No Hit
CTACCACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC12570.12902709261004255No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12540.12871915205488735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12530.12861650520316892No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12360.12687150872395592No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA12240.125639746503335No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12170.12492121854130611No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC12110.12430533743099566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12100.12420269057927724No Hit
CAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATA12070.12389475002412202No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG12070.12389475002412202No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11960.12276563465521949No Hit
TCCCAGTCCATGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG11820.12132857873116173No Hit
ACATAGTACTTCTCACCTCCATCTTCCTTGATGTTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC11580.11886505428991988No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11470.11773593892101736No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG11410.11712005781070688No Hit
GTATTCAATGAGCCAGTCAAAATATCCTATAGTCGCACAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTT11380.11681211725555166No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11230.11527241447977549No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA11190.11486182707290185No Hit
TGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAG11100.11393800540743615No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10870.11157712781791268No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10770.11055065930072859No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT10710.10993477819041812No Hit
GACATGGACTGGGAAGCATACTTGATGAGGAGTTTTGGAGACTGATCTGG10710.10993477819041812No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10700.1098321313386997No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC10660.10942154393182607No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10610.108908309673234No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10610.108908309673234No Hit
GTGAAGGGCCGACTCACCATCTCCAGAGACAACGCCAAGAATTTATTGTA10560.10839507541464195No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10550.10829242856292354No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCC10480.10757390060089467No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10340.10613684467683691No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10300.10572625726996328No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10270.10541831671480803No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA10200.10469978875277917No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC10170.10439184819762393No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10140.10408390764246869No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10120.10387861393903189No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10070.10336537968043981No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC10020.10285214542184776No Hit
GTGTGAGGTGCAGCTTGTGGAATCGGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC9880.10141508949779No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA9840.10100450209091637No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9840.10100450209091637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC252.5477115E-455.2843063
CGTATGC408.005427E-751.98914741
TCGTATG408.025363E-751.97041340
ATCGTTA200.00731740251.82371510
ACGAGAC451.839051E-646.07971222
GTGGTAT2050.045.5271151
GTATCAA14350.041.19121
GGTATCA5800.040.5266881
CTCGTAT557.185805E-637.79277839
TGGTATC2300.037.5572742
TATCAAC16350.036.3490072
CTTATAC601.3246508E-534.5668831
ATCAACG17450.033.8596533
TCAACGC17550.033.666724
CAACGCA17900.033.0084345
AACGCAG18200.032.6541866
TTATACA652.2970724E-531.8947932
CGCAGAG19050.031.0126198
ATGTCAG21100.030.96264870
CATCGTA450.00459027530.710359
Produced by FastQC (version 0.11.5)