FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974214
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAACTCCTCAT47590.48849636732791774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33210.3408901945568428No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29710.3049637964553989No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25540.2621600592888215No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24010.2464550909759047No Hit
GTATTACTGTCATCAGAGTGGTAGTTTGCCGTGGACGTTCGGCCAAGGGA21210.2177139724947496No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20890.21442927323976047No Hit
GTCCATGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGC19710.202316944736988No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19700.20221429788526957No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19580.20098253566464863No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18980.19482372456154395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18500.18989667567906024No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18280.1876384449412552No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17600.18065845902440328No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17260.1771684660659773No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATGACTAC17230.17686052551082204No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16820.17265200459036723No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG16810.1725493577386488No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16740.17183082977661993No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16580.17018848014912535No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16470.16905936478022282No Hit
GTGTAAGCTAGTACCAATGTTCTGACTGGCCCGGCAGGTGATGGTGACTC16360.1679302494113203No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16290.16721172144929142No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16280.16710907459757302No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15800.1621820257150893No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15490.15899997331181853No Hit
CAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA15390.15797350479463446No Hit
GTCTCAAGACTTTCAGTCTGTGGCTCCAAAGGAGAGAGTCACCATCACCTGCCGGGCCAGTCAGAACATTGGTA15310.15715232998088716No Hit
GAGTGGTAGTTTGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT15220.15622850831542145No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15110.15509939294651892No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14850.15243057480184025No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14210.14586117629186196No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14070.14442412036780422No Hit
GTACTATGTGGGCTCTGTGAAGGGCCGACTCACCATCTCCAGAGACAACG14060.1443214735160858No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAACGTATTACTGTCATCAGAGTGGTAGTTTG13750.14113942111281505No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13640.14001030574391252No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13540.13898383722672844No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG13480.13836795611641795No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13370.13723884074751544No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13340.1369309001923602No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13250.1360070785268945No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13210.13559649112002087No Hit
CTCCTGTGAAGCCTCTGGATTCACCTTTAGTAGTCATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG12730.13066944223753713No Hit
ATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA12650.12984826742378985No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC12520.12851385835145052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12430.1275900366859848No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12340.1266662150205191No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA12330.1265635681688007No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12220.12543445279989818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12010.12327886891381154No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11980.12297092835865632No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCA11970.1228682815069379No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11810.12122593187944332No Hit
GTGTGAGGTGCAGCTTGTGGAATCGGGGGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGAAGC11540.11845446688304621No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11530.11835182003132783No Hit
TCCCAGTCCATGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG11530.11835182003132783No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC11230.11527241447977549No Hit
CAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATA11140.11434859281430979No Hit
GTATTCAATGAGCCAGTCAAAATATCCTATAGTCGCACAATAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTT11130.11424594596259138No Hit
CTACCACTCTGATGACAGTAATACGTTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC11110.11404065225915457No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11000.11291153689025205No Hit
GAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTCCTTGT10960.1125009494833784No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10850.11137183411447588No Hit
TGTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAG10720.11003742504213654No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10560.10839507541464195No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10540.10818978171120512No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA10510.1078818411560499No Hit
ACATAGTACTTCTCACCTCCATCTTCCTTGATGTTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC10440.10716331319402103No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10430.10706066634230262No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA10390.10665007893542898No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA10390.10665007893542898No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG10330.10603419782511851No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10320.1059315509734001No Hit
GACATGGACTGGGAAGCATACTTGATGAGGAGTTTTGGAGACTGATCTGG10200.10469978875277917No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA10170.10439184819762393No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC10150.10418655449418711No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC10010.10274949857012936No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9810.10069656153576113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9800.10059391468404272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2550.041.985472
GTGGTAT2700.040.969961
GGTATCA6250.040.928991
GTATCAA14100.038.981391
TATCAAC15350.035.3239062
TATATCT400.002596435434.4992035
ATCAACG16300.033.2429473
TCAACGC16800.032.0333334
CAACGCA17250.030.9992855
ATGTCAG19850.030.29096670
AACGCAG17700.030.211176
TTCGGGG10100.029.76612370
CAGAGTA18950.028.58245710
AGAGTAC18950.028.22124511
CGCAGAG18950.028.218358
ATTTAAG500.00744885627.8135152
GCTTAGC500.00765994227.6547241
CTGAAAG8500.027.26372770
GCAGAGT19900.027.2179649
AACCCGA658.15342E-426.8117258
Produced by FastQC (version 0.11.5)