FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865562
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.040.0815851
GGTATCA8100.039.2853281
CAACGCA20550.032.805725
TATCAAC21000.032.598512
TCAACGC20850.032.499514
ATCAACG21000.032.4338723
AACGCAG21200.031.7998856
ACGCAGA22850.029.501917
AGAGTAC22900.028.6826911
GTATAGA854.339432E-628.4843751
CGCAGAG23700.028.4438238
GTGGTAT3350.027.8770331
CAGAGTA23850.027.39524510
TAACGGC1306.0663297E-926.63399336
GCAGAGT25850.026.078099
TGGTATC3550.024.3480152
ACGGGTA1851.8189894E-1124.29396416
TTAACGG1352.5685586E-723.08145935
CGAACTA1553.979585E-822.31236829
AGTACGG22150.022.16365813
Produced by FastQC (version 0.11.5)