FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809362
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33410.41279427499684934No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGACTTGGATCCG22290.2754021068446505No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21840.26984217198237626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21100.2606991679866364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20830.25736320706927185No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19640.24266026821125777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19550.24154828123880293No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18720.2312932902706082No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18530.22894576221764795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18390.22721600470494044No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18340.22659823416468777No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18220.22511558486808125No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17960.22190317805876728No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17950.22177962395071674No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17740.21918498768165542No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAA17610.21757878427699842No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17380.21473703979183606No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16560.2046056029316919No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT16320.20164030433847896No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15600.19274440855884017No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGA15600.19274440855884017No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15520.19175597569443587No Hit
GATCTATGGTGCAGCCACTTTGCAACTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA15030.18570182439995947No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14800.18286007991479708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14630.18075966007793792No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14610.18051255186183687No Hit
GGCTAACAGTTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14470.17878279434912933No Hit
ACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAATCTGA14390.177794361484725No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAAT14370.17754725326862392No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14270.17631171218811856No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC13420.16580961300382274No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12870.15901413706104314No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12710.15703727133223452No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12040.14875914609284843No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11960.14777071322844415No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11710.14468186052718068No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC11630.14369342766277637No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11500.14208722425811934No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11450.14146945371786668No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11380.1406045749615129No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11370.14048102085346237No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11350.1402339126373613No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11300.13961614209710857No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11290.13949258798905806No Hit
GAGTAATCTCGGACCACCACATCTCTGGCACAAAAATACACGGCCGTGTC11140.13763927636829998No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11140.13763927636829998No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10930.13504464009923867No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10920.13492108599118813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10830.13380909901873325No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10600.1309673545335709No Hit
GTAGTAGGTGGTCCCACTGTAATGGATGTTCCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAAGT10170.12565452788739773No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACTTT10150.12540741967129665No Hit
AGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAA10100.12478964913104397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9830.12145368821367941No Hit
GGGTTCCCTGGCCCCAGTAGTCGTCTACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGG9820.12133013410562889No Hit
ACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGGC9800.1210830258895278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTCGAGGGAAA9600.11861194372851704No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9470.11700574032386002No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTC9390.11601730745945571No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC9380.11589375335140518No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9350.11552309102725357No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9330.11527598281115248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9220.11391688762259655No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAG9210.11379333351454603No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9120.11268134654209118No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9030.11156935956963634No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8940.11045737259718147No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA8880.10971604794887825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8870.10959249384082771No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA8660.1069978575717664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8640.10675074935566534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8610.10638008703151372No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8610.10638008703151372No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8410.10390900487050295No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8300.10254990968194702No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8300.10254990968194702No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8270.1021792473577954No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC8220.10156147681754271No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8150.10069659806118893No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTG8120.10032593573703731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1650.043.947591
GTATCAA13900.042.7280731
TATCAAC15400.038.1154562
ATCAACG16100.036.6704563
TCAACGC16100.036.456014
CAACGCA16450.035.6803475
AACGCAG16800.035.1403476
GTAGTTT951.9373055E-434.26009870
CGCAGAG18100.032.6124278
AGAGTAC18250.031.96608211
GCAGAGT19000.031.0676239
ACGCAGA19700.029.7884737
AGTATGA500.007264760327.955962
CGGGATA1705.456968E-1226.3989617
AGTACGG17700.026.32849513
TATCTCT1057.6285505E-726.3055084
TTCGGGG10900.026.27655270
GAGTACG17750.026.25433212
CAGCGCT3600.025.8944854
ACGGGGG3350.025.76088316
Produced by FastQC (version 0.11.5)