FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809362
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33520.4141533701854053No Hit
GTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGACTTGGATCCG22180.27404301165609457No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21700.2681124144696687No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20120.2485908653976836No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19510.24105406480660077No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19240.23771810388923623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.22548624719223287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18230.22523913897613182No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18050.22301516503122212No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT17530.21659035141259414No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17090.21115397065837044No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16890.20868288849735964No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16680.20608825222829832No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16530.20423494060754027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16180.1999105468257714No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16160.19966343860967034No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT15580.1924973003427391No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAA15260.18854356888512186No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15210.18792579834486917No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15180.18755513602071755No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14900.18409562099530247No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTGGTGATTACTACTGGA14710.18174809294234223No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAGAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC14580.18014188953768526No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13870.17136954786609698No Hit
GGCTAACAGTTTCCCTCGAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13580.1677864787326314No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAAT13370.16519184246357008No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13320.16457407192331738No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13160.16259720619450876No Hit
GATCTATGGTGCAGCCACTTTGCAACTTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA13100.16185588154620553No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12960.160126124033498No Hit
ACTACACCCCGTCCCTCAAGAGTCGACTTACAATATCCGTAGAAACGTCCAAGGACCAGTTCTCCCTGAATCTGA12870.15901413706104314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12550.1550604056034259No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12390.15308353987461729No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12260.15147733646996028No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12110.1496240248492022No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11610.14344631944667527No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT11450.14146945371786668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11230.13875126334075483No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11120.1373921681521989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11060.13665084350389567No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10980.13566241063949136No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10860.1341797613428849No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10820.13368554491068274No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10790.13331488258653112No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10590.13084380042552035No Hit
GAGTAATCTCGGACCACCACATCTCTGGCACAAAAATACACGGCCGTGTC10580.1307202463174698No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10490.12960825934501496No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10460.12923759702086335No Hit
GTAGTAGGTGGTCCCACTGTAATGGATGTTCCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAAGT10210.12614874431959988No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10200.12602519021154934No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC10140.1252838655632461No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10090.12466609502299342No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10090.12466609502299342No Hit
GGGTTCCCTGGCCCCAGTAGTCGTCTACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGG10020.12380121626663966No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9950.12293633751028588No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGAC9920.12256567518613426No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9590.11848838962046648No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9450.11675863210775896No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9390.11601730745945571No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9330.11527598281115248No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9240.11416399583869764No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9170.11329911708234389No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9130.11280490065014172No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9080.11218713010988901No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTC8940.11045737259718147No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8890.1098396020569288No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTCGAGGGAAA8860.10946893973277719No Hit
AGTCAAGGTGTTGCCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAA8820.10897472330057502No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8780.10848050686837288No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8730.10786273632812017No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8730.10786273632812017No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8700.10749207400396855No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA8620.10650364113956425No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTCTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACTTT8610.10638008703151372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8570.10588587059931155No Hit
ACCCAATAATGTCCCGAACCAGAGTAATCTCGGACCACCACATCTCTGGC8430.10415611308660401No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8250.10193213914169433No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8230.10168503092559324No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAAGGTGTTGCCAG8210.10143792270949216No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG8160.10082015216923948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAGT200.007372517651.724658
GTGGTAT1950.044.280241
GTATCAA12500.041.169991
TTTATAG350.001349600839.4165653
TATCAAC14200.036.209822
ATCAACG14700.034.958743
CGGGATT601.3425848E-534.4980217
TCAACGC15050.034.137314
CAACGCA15650.033.0508965
AACGCAG15750.032.841056
ATACTGC553.265551E-431.3482726
GTATAAT450.00459668530.7009621
ATTTATA450.00461620230.6743872
CGCAGAG17100.029.8450228
AGAGTAC17000.029.81774111
GCAGAGT17650.028.9150099
CGAAATT605.2427006E-428.91456648
ACGGGAT2250.027.59671216
GACCGCT500.007752100527.586487
AATAGCG906.25121E-627.17785862
Produced by FastQC (version 0.11.5)