FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273260
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4850.041.2330931
GTATCAA29750.037.318881
GGTATCA13500.036.522621
TATCAAC35650.031.1353442
TGGTATC6600.030.2929362
ATCAACG36600.030.2330023
CAACGCA37000.029.9061585
TCAACGC36950.029.7600444
AACGCAG38550.028.7037076
ACGCAGA41900.026.4067047
AGAGTAC42550.025.92230611
CGATTGT1200.001090536925.5074570
CGCAGAG43950.025.0965658
CAGAGTA44350.024.79249610
GCAGAGT47250.023.343799
TAACGGC2700.023.0242536
AGTACGG39950.022.95028313
GAGTACG41100.022.39198712
TTAACGG2800.022.19671635
TTTAACG2850.021.80472834
Produced by FastQC (version 0.11.5)