FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454511
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18060.12416544116888768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.037.7933651
GTATCAA26650.036.6735271
CGCGTAT400.002587925234.52344512
TATCAAC29950.032.855412
ATCAACG32400.030.2644123
TCAACGC32700.030.0923444
CAACGCA33200.029.6391475
GTGGTAT5450.029.1490751
TCTAGTA4050.028.995626
TGGTATC5750.028.8225442
AACGCAG34750.028.3171126
CTAGTAG4300.027.30981327
TGGATTG8500.026.15255270
ATGGTCG4800.025.8952525
AGAGTAC38100.025.7340111
ACGCAGA38450.025.6793387
TATGGTC5150.025.4762364
CAGAGTA39050.025.19636710
CGCAGAG39300.025.1239328
GTCGGTC4850.024.2020028
Produced by FastQC (version 0.11.5)