FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454511
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16310.11213390617190244No Hit
ATGTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT14600.100377377689134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31600.038.2695271
GTGGTAT5700.038.188961
GGTATCA14750.035.840291
TGGTATC6250.035.3616752
TATCAAC35600.033.6598662
ATCAACG37150.032.320633
TCAACGC37600.032.0145654
CAACGCA38500.031.1765825
AACGCAG38900.030.944676
ACGCAGA42600.028.2569877
AGAGTAC42800.027.88605911
CAGAGTA43500.027.3561410
CGCAGAG44100.027.1394428
CTAGTAG5000.026.93379827
TGGATTG8500.026.60665370
TCTAGTA4950.026.50644726
GCAGAGT46250.026.0269769
TTCTAGT5300.025.40399725
ATCCGTC5350.024.6008540
AGTACGG40050.024.29438813
Produced by FastQC (version 0.11.5)