FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.039.0168151
GGTATCA8600.036.9563371
TCAACGC21150.032.1744084
CAACGCA21450.031.7244195
ATCAACG21450.031.5633813
AACGCAG21950.031.0017686
TATCAAC22650.029.8911482
ACGCAGA23800.028.590497
AGAGTAC23950.028.12298611
TGGTATC3800.027.2704562
GTGGTAT3850.026.9190671
CGCAGAG25300.026.8954038
CAGAGTA25100.026.83448210
TTTCGAT1659.447793E-625.76777870
GCAGAGT26850.025.4714269
GTAGTTT4300.025.42534870
AGTACGG23900.024.27972413
GAGTACG23950.024.08481412
ACCGATC750.001940899523.10460742
CGTAATC1103.1664167E-522.04299741
Produced by FastQC (version 0.11.5)