FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741445
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.037.165311
GTATCAA49050.036.6134641
GTGGTAT9450.032.7657431
ATCAACG57400.031.463493
TCAACGC57650.031.2673744
CAACGCA59100.030.3829565
AACGCAG60150.030.0813356
TATCAAC60650.029.8342022
ACGCAGA65950.027.4848217
CTCGTAT906.7372803E-626.92027939
TGGTATC11350.026.6714292
CGCAGAG67700.026.6219438
AGAGTAC67050.026.52093711
CAGAGTA68800.025.89634310
GCAGAGT73150.024.8265699
TTTAGCG851.4666804E-424.29636426
TCGTATG1001.5190821E-524.24156240
AGTACGG58650.023.04738413
GAGTACG59850.022.75768712
GTAGTTT9100.021.31372670
Produced by FastQC (version 0.11.5)