FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741445
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20920.12013012182411732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52850.037.309181
CCGTATA400.002629536734.4116971
GGTATCA24000.033.4080241
GTGGTAT11500.032.017841
ATCAACG61800.031.6459453
TATCAAC62550.031.396242
TCAACGC62300.031.376644
CAACGCA64400.030.4593015
AACGCAG65800.029.8634416
TGGTATC12750.028.5933152
ACGCAGA69550.028.2532637
AGAGTAC70400.027.52491811
CGCAGAG71700.027.4060578
CAGAGTA72600.026.97167810
GCAGAGT77050.025.5476919
AGTACGG64150.023.30113
GAGTACG65400.023.06448712
ATAGCGG5650.020.62628663
CTTACCG850.004223880420.2078829
AATAGCG5950.020.1512862
Produced by FastQC (version 0.11.5)