FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063739
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.043.129911
GTATCAA21150.040.6216471
ATCAACG26100.033.0093353
TCAACGC26250.032.8129964
CAACGCA26850.032.3363845
TATCAAC27450.031.5262952
AACGCAG27700.031.468496
GCGTTAC450.004614640530.67738330
ACGCAGA29500.029.548387
CGCAGAG30150.028.9113528
CAGAGTA30150.028.68280410
AGAGTAC30350.028.38160111
CGATAAG757.19321E-427.39267269
GCAGAGT32700.026.6555449
CGAACGG950.00989786626.23706270
AGTACGG26100.025.34759513
GAGTACG26300.025.02264412
TGGTATC3750.024.8241312
TCGATAA750.001297568124.75932168
GTGGTAT4450.024.0364461
Produced by FastQC (version 0.11.5)