FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924966
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50250.039.2691271
GGTATCA22350.039.068941
TATCAAC60350.032.6325652
ATCAACG62750.031.6027363
TCAACGC63750.031.215644
CAACGCA64200.030.9968385
GTGGTAT8850.030.687341
AACGCAG65300.030.4746846
ACGCAGA70050.028.5034037
CGCAGAG72650.027.5779328
TGGTATC10100.027.5636982
AGAGTAC72350.027.02658711
CAGAGTA73100.026.7499910
GCAGAGT78550.025.4627639
CGTATGC1401.33113645E-824.7418641
AGTACGG61650.024.24787313
GAGTACG62350.024.0307612
CTCGTAT1553.9946826E-822.30777239
TCGTATG1605.545371E-821.62704540
GTACGGG73950.020.02887314
Produced by FastQC (version 0.11.5)