FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924966
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22600.11740467104354052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55200.037.680851
GTGGTAT11050.033.602061
GGTATCA25700.032.908371
TATCAAC66300.031.3890112
ATCAACG66300.031.0022473
TCAACGC67500.030.5940654
CAACGCA68450.030.269695
AACGCAG68450.030.1694586
ACGCAGA72400.028.5234727
CGCAGAG73700.028.0210728
AGAGTAC73250.027.7753311
TGGTATC13250.027.4731542
CAGAGTA74600.027.3604110
GCAGAGT79550.026.0028889
AGTACGG65900.024.26878513
GAGTACG67000.023.86662112
TACGGGC14050.020.76462415
CTCGCTA1357.117542E-620.3462523
GTACGGG77200.020.31638714
CGATACC1055.580237E-419.93997650
Produced by FastQC (version 0.11.5)