FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273474
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15760.12375596203770159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32700.037.526451
GGTATCA16250.035.84831
GTGGTAT7500.031.7119621
ATCAACG40100.030.3900133
TATCAAC40800.029.9800722
TCAACGC41000.029.7975164
CAACGCA42100.029.1007045
TGGTATC7650.028.8259092
AACGCAG43900.027.9858996
ACGCAGA45750.026.8542297
AGAGTAC46250.026.11848411
CGCAGAG47300.026.0469868
CAGAGTA47000.025.77391410
CGAACTA3650.025.49599629
GCAGAGT51650.023.919929
AGCGAAC4150.022.4118527
AGTACGG41600.022.34129513
GAGTACG41750.022.17596412
GCGAACT4250.021.89139628
GTGTATA2453.8198777E-1119.696871
Produced by FastQC (version 0.11.5)