FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783505
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA9570.12214344515989049No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9480.12099476072265014No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT7840.1000631776440482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17650.042.12891
GGTATCA7050.036.7924351
CAACGCA21100.034.412195
ATCAACG21250.034.331993
TCAACGC21350.034.0092354
AACGCAG22400.032.5694126
TATCAAC22950.031.9395372
ACGCAGA22950.031.6301467
AGAGTAC23450.030.0712811
CAGAGTA23900.029.64971710
CGCAGAG25200.028.6688568
TTAACGG3100.027.91777435
CTACGCT500.007643634527.66614328
GCAGAGT26600.027.5498229
TAACGGC3250.026.63436936
GTGTAAT658.531316E-426.6037581
AGTACGG23150.025.5334613
TTTAACG3450.025.08233334
ATACCGT700.001316466124.6971846
GAGTACG24000.024.6291512
Produced by FastQC (version 0.11.5)