FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952099
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44740.46990911659396767No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA34790.36540317761073166No Hit
GGCCAAAAGTGTGCGGGGTACTGTAAGTCTGTTGACAGAAGTAAGTTGCA33960.35668559677092404No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31180.32748695251229126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29420.3090014798881209No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27700.29093613164177257No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27250.28620973239127445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26450.2778072448348334No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25630.26919469508948124No Hit
GTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT24620.2585865545494744No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24510.25743121251046375No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA23710.24902872495402265No Hit
GGGTACTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT23540.2472431963482789No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAAGTATTTGAATTGGTATCACCAGAAA23240.24409226351461352No Hit
CTGTAAGTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC23180.2434620769478804No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23020.24178157943659223No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG22450.23579480705262792No Hit
GACTTACAGTACCCCGCACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT21640.22728728840173135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21060.22119548492331156No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20660.216994241145091No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20620.21657411676726895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20270.21289802846132597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19550.205335789660529No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18790.19735342648190995No Hit
ACAGTACCCCGCACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGA18080.1898962187755685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18070.189791187681113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18010.18916100111437992No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17240.18107360684130538No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16700.17540192774070765No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15780.16573906705080038No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGTGCGGGGTA15680.16468875610624525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15640.1642686317284232No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15360.16132776108366884No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15240.16006738795020264No Hit
AGTCAGAGCATTACCAAGTATTTGAATTGGTATCACCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATAAATG15070.15828185934445893No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCTCC15030.15786173496663689No Hit
GTATTTGAATTGGTATCACCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA14750.15492086432188248No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14680.1541856466606939No Hit
GTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT14520.15250514914940566No Hit
CCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG14360.15082465163811745No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13740.14431272378187562No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT13600.14284228845949845No Hit
CTTACAGTACCCCGCACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAA13310.13979638672028855No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACC13170.13832595139791135No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA13080.13738067154781172No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13050.1370655782644452No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12790.13433476980860184No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12770.1341247076196908No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12730.13370458324186876No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA12700.13338948995850222No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12470.1309737747860254No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12390.1301335260303813No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12330.1295033394636482No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11960.12561718896879423No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11950.1255121578743387No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAAC11900.12498700240206113No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT11770.12362159817413945No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11720.12309644270186187No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11620.12204613175730676No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11600.12183606956839572No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT11520.12099582081275162No Hit
GATAAATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11470.12047066534047406No Hit
GAGCATTACCAAGTATTTGAATTGGTATCACCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATAAATGCTGCATC11140.1170046392234421No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11030.11584929718443146No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10710.11248830216185501No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10480.1100725869893782No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG10430.10954743151710064No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10380.10902227604482308No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10380.10902227604482308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10380.10902227604482308No Hit
CCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATAAATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT10370.10891724495036756No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10360.10881221385591205No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTTCTGTCAACAGACTTACAGTACCCCGCACACTTTTGGCCAGGGGA10340.10860215166700102No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATTTATCAGGAGC10230.10744680962799036No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9840.10335059694422534No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTACCAAGTATTTGAATTGGTATCACCAGAAACCAGGGAAAG9720.10209022381075918No Hit
GTAGTAAACGGCCGTGTCCTCAGATCTCAGGCTGGTCACCTCCATGTAGG9700.10188016162184815No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG9670.10156506833848161No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9630.10114494396065955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)