FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721802
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18450.041.5990831
GGTATCA7950.038.703481
TATCAAC21750.035.7519652
TCAACGC22700.034.255744
CAACGCA23100.033.6625675
ATCAACG23200.033.5174673
AACGCAG23600.032.949386
ACGCAGA24300.031.9980017
AGAGTAC26000.029.37416511
CGCAGAG26500.029.3415648
CAGAGTA26600.028.7115910
GCAGAGT27750.027.7708039
GTATAAG2900.025.035051
GAGTACG24800.023.96753512
AGTACGG25050.023.72833813
GAGTACT7750.021.84947412
CATTACG800.002864599221.60613324
TTAATCG2251.0913936E-1121.5280137
TATTAGA2101.0004442E-1021.3944322
CGAACCT2451.8189894E-1221.30990853
Produced by FastQC (version 0.11.5)