FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721802
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.041.5863381
GGTATCA7400.040.1367721
TATCAAC22900.034.5027732
ATCAACG22950.034.413293
TCAACGC23400.033.7374654
CAACGCA23700.033.310415
AACGCAG23700.033.310416
AGAGTAC24950.031.6481311
CAGAGTA25200.031.32981910
ACGCAGA25250.031.2656127
GTGGTAT3350.029.8970931
CGCAGAG26800.029.3287078
GCAGAGT27800.028.6457399
AGTACGG24700.024.43342613
GAGTACG24900.024.37398312
ACACCGT1001.5717318E-524.1318636
TATACTG1151.7073107E-623.9819775
TGGTATC4100.023.562872
CGAACTA2350.023.50428829
AATAGCG1854.0563464E-1022.69007362
Produced by FastQC (version 0.11.5)