FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942844
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34100.041.3193931
TCAACGC39800.034.8739934
GGTATCA15900.034.7512971
ATCAACG40100.034.69833
CAACGCA40350.034.3977475
AACGCAG42850.032.4714556
TATCAAC43300.032.2145582
ACGCAGA44800.030.977027
AGAGTAC47100.029.53687911
CGCAGAG47300.029.3397628
CAGAGTA48050.028.80995810
GCAGAGT53000.026.2494879
TTAACGG5650.025.7077735
TAACGGC5700.025.48554836
GTATAAG6150.025.2688561
TTTAACG5800.025.03581434
AGTACGG45050.024.82736613
GTAGTTT10050.024.26554570
GAGTACG46350.024.13102312
GTGGTAT5750.024.0237221
Produced by FastQC (version 0.11.5)