FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049944
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA46050.2246402828565073No Hit
GTGTATTACTGTGCGAGACTCACCCTTCTAATGATTTTTGGAGTGGCTCCAAACGACTACTGGGGCCAGGGAATC36800.1795170990036801No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35690.17410231694134085No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30570.1491260249060462No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30220.147418661192696No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27560.13444269697123434No Hit
GTATTACTGTCAGCAGTATGGTACCTCATTGACGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26820.13083284226300818No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26410.12883278762736933No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24210.11810078714345366No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24050.1173202780173507No Hit
GTATGGTACCTCATTGACGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22930.11185671413463001No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22360.10907615037288823No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA20950.10219791369910593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT14600.041.55456570
GGTATCA21700.040.944431
GTATCAA48400.037.7819181
TCGTATG1800.034.58797540
TATCAAC54550.033.4502532
CTCGTAT1900.032.75471539
ATCAACG56000.032.522653
TCAACGC56250.032.3781054
CGCTAAT1950.031.83608232
CAACGCA57700.031.743455
AACGCAG57950.031.6065066
TCGCTAA2050.030.27274531
AGAGTAC61850.029.66702711
CGCAGAG63400.028.9960298
CTACGGA15000.028.49563469
CAGAGTA66100.027.7074610
GCAGAGT66550.027.6752939
ACGCGTA500.007761199527.58183531
ACGCAGA67550.027.1127017
CGTATGC2300.027.08743941
Produced by FastQC (version 0.11.5)