FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049944
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA44620.21766448254196213No Hit
GTGTATTACTGTGCGAGACTCACCCTTCTAATGATTTTTGGAGTGGCTCCAAACGACTACTGGGGCCAGGGAATC39020.19034666312835863No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37130.18112689907626744No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT33650.16415082558352814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29530.14405271558637697No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27820.13571102430115164No Hit
GTATTACTGTCAGCAGTATGGTACCTCATTGACGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26530.12941816947194654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24280.11844225988612371No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23900.11658855071162919No Hit
GTATGGTACCTCATTGACGTGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23530.11478362335751612No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22700.11073473226585703No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21830.10649071389267219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.038.496971
GTATCAA48300.037.3728941
TACGGAT16800.036.51035770
TATCAAC56400.032.0399632
TCAACGC56850.031.511854
ATCAACG57300.031.3935813
CAACGCA58850.030.4394455
AACGCAG59350.030.1830046
GTGGTAT10550.028.7344421
AGAGTAC63900.027.98069811
CGCAGAG65950.027.1102818
CAGAGTA67100.026.69689210
GCAGAGT67300.026.5664639
ACGCAGA68400.026.3402987
TGGTATC11650.025.7042622
CTACGGA17050.025.3470469
AGTACGG54650.024.4775813
GAGTACG55300.024.12709612
TATATCG750.002033271622.9220895
CCGCTAT2753.2268872E-822.75970370
Produced by FastQC (version 0.11.5)