FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329572
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG652.0736479E-1047.91928540
CTCGTAT605.458787E-946.12331439
CGTATGC704.2382453E-1044.53738441
GTATCAA9150.039.031591
TCTCGTA753.9493898E-836.85917738
TTATACA853.1250238E-936.3216932
GGTATCA4000.036.0573121
TCAACGC10650.035.1090774
GTATAAT400.002653341534.3402981
TATACAC1004.0199666E-1034.303823
TATCAAC10900.033.6743972
CAACGCA11250.033.5415155
ATCAACG11300.033.089533
AACGCAG11500.032.812356
TCGGTTG652.119684E-532.2622451
GAACGGT552.7705455E-432.2318162
CAATACA450.004748580530.492294
ACGCAGA12400.030.430817
AGAGTAC12050.030.1759811
CGCAGAG12600.029.9477838
Produced by FastQC (version 0.11.5)