FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329572
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6950.21087956501159077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT300.002998791964.899670
CGTACTT555.5462105E-447.19970370
GTATCAA8950.039.4797331
GGTATCA3600.039.0698131
ACTACTC501.9473166E-434.2271275
TATCAAC10750.032.8541832
ATCAACG11050.032.233333
TGGTATC1600.032.146462
TCAACGC11200.031.4767364
CAACGCA11250.031.3368365
GTGGTAT1650.031.1865461
AACGCAG11600.030.3913296
ACGCAGA12200.028.8966717
ATACTGA605.6713895E-428.5226066
AGAGTAC12600.027.97931911
CAGAGTA12600.027.97931910
CGCAGAG12900.027.5939648
AATAGCG657.3572295E-427.28054262
GCAGAGT13600.026.1736879
AGTACGG11600.025.67034513
Produced by FastQC (version 0.11.5)