FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829421
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27560.3322799880880759No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16560.19965735133303836No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16160.19483470999649152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15680.18904754039263535No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14710.17735263515150929No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14620.17626754085078628No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14340.17289169191520348No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13950.16818961661207035No Hit
GATCTATGCTGCATCCCGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG12900.15553018310363492No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12590.15179263606781118No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12510.1508281078005018No Hit
GTAGTAGATTGCAGAATCAGCAGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCCA12420.14974301349977875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12180.14684942869785067No Hit
ATCTACTACTGTCAACAGAGTGACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA12160.14660829663102332No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11420.1376864101584117No Hit
ATGCTGCATCCCGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCA11040.13310490088869223No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11000.13262263675503755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10920.1316581084877282No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10830.13057301418700515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10280.12394188234925328No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTACCTATTTAAATTGGTATCAGCAGAAA10150.12237452391487555No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9850.11875754291246544No Hit
ACTGTCAACAGAGTGACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTG9670.11658735431101938No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9580.11550226001029633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9540.11501999587664165No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTAC9300.11212641107471358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9080.10947395833961282No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9050.10911226023937179No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8840.10658037353768472No Hit
GAGTGACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8790.10597754337061636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8650.10428961890282498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8560.10320452460210194No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGAGA8430.10163716616772424No Hit
GTCTGCAACCTGCTGATTCTGCAATCTACTACTGTCAACAGAGTGACAGTCTCCCGTGGACGTTCGGCCAAGGGA8400.10127546806748323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23900.040.234481
TATCAAC27050.035.534122
GTGGTAT3050.033.901081
TCAACGC28550.033.6326754
ATCAACG28550.033.518193
AACGCAG29700.032.6780366
CAACGCA29650.032.268845
AGAGTAC31500.030.59589411
ACGCAGA32000.030.4368557
CGCAGAG32100.030.234828
CAGAGTA33850.028.57004510
GCAGAGT33750.028.5527239
ACGGGTA500.0078098827.5446116
GCGTTGT3200.027.42672362
GAGTACT9950.027.33203912
AGTACTT10500.026.56087113
CGTTGTC3350.026.23415263
GCGTAGA12500.025.58063770
GAGTACG28150.024.58021512
AGTACGG28200.024.4189813
Produced by FastQC (version 0.11.5)