FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461448
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGATTCTTGGACGTGTCTACTGA25030.17126849535529148No Hit
GTCTCTGGTGGCTCCATCAGTAACTATTACTGGACCTGGATCCGGCAGCC22530.15416217340610136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17280.1182388973128021No Hit
GTCTATTACTGTGCGAGACACGGGGGACTTTATGGAGTGGAAACTGTTTG17170.11748621914703773No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15690.10735927655311718No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15290.10462226504124676No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15270.10448541446565324No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14990.10256950640734395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26950.038.711921
TATCAAC31950.032.7506332
TCAACGC33850.030.8113734
ATCAACG33850.030.7082963
CAACGCA34800.030.1697675
AACGCAG35100.029.8135136
GGTATCA16950.027.3106231
TGGATTG12250.027.14021770
ACGCAGA38700.026.9472337
AGAGTAC39050.026.6163711
GACGTGT9000.026.42792162
CGCAGAG40100.026.006438
GTGTCTA8850.025.79026465
GTCTACT9400.025.73865367
CAGAGTA40550.025.63266810
TGTCTAC9550.025.46633366
TCTACTG10200.024.98025368
GCAGAGT42150.024.7415879
ACGTGTC9250.024.6048163
TAGACGG9550.024.5913945
Produced by FastQC (version 0.11.5)