FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179998
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8980.49889443216035734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5800.32222580250891675No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAA5570.30944788275425283No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5450.302781142012689No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG5310.29500327781419794No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT4870.2705585617617974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4730.26278069756330624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4670.25944732719252434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC4610.25611395682174243No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT4430.24611384570939676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4310.23944710496783295No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC4050.2250025000277781No Hit
AGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAACCAGGGAA3630.2016689074323048No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3480.19333548150535007No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT3470.1927799197768864No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG3370.18722430249224992No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG3300.18333537039300435No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3250.18055756175068613No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA3140.17444638273758598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3040.16889076545294948No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2950.16389070989677662No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT2850.15833509261214015No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC2850.15833509261214015No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC2790.15500172224135825No Hit
ACATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCA2790.15500172224135825No Hit
ATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2660.1477794197713308No Hit
GGCCAAAAGTGTACGGAGGGAGATTATCATAGTGTTGACAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTG2650.14722385804286714No Hit
ATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA2610.14500161112901255No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2540.14111267902976699No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2520.1400015555728397No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2510.13944599384437603No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC2480.1377793086589851No Hit
GTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA2480.1377793086589851No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2390.13277925310281224No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2380.1322236913743486No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2380.1322236913743486No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT2370.13166812964588495No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGA2230.12389026544739387No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2230.12389026544739387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2210.12277914199046655No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2160.1200013333481483No Hit
CCCCTAAACTCCTGATCTACGATGCATCCATTTTGGAAACAGGGGTCCCA2100.11666796297736641No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2070.11500127779197546No Hit
CTATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2070.11500127779197546No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAC2040.11333459260658452No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC2030.11277903087812087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1960.10889009877887532No Hit
GAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAACCAGGGAAAG1950.10833453705041167No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA1940.10777897532194802No Hit
GATCTACGATGCATCCATTTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGACGTGGATCTGGGACAGATTTTA1910.10611229013655708No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCATCCATTTTGGAAACAGGGGTCCCATCAAGGTT1890.10500116667962976No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC1870.10389004322270247No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT1850.10277891976577518No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA1810.10055667285192058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)