FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179998
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9990.555006166735186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5700.3166701852242803No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG5240.29111434571495237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5150.28611429015877954No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5120.2844476049733886No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAA4480.248891654351715No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAT4270.2372248580539784No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4260.23666929632551473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4250.23611373459705107No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4240.23555817286858743No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG4020.22333581484238713No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC3700.20555783953155035No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC3630.2016689074323048No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT3500.19444660496227734No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT3350.18611317903532262No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG3290.18277980866454072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3270.18166868520761342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3210.17833531483683152No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3030.16833520372448582No Hit
AGTCAGGACATTAACATCTTTTTAAGTTGGTATCAGCAGAAACCAGGGAA3010.16722408026755853No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG2930.16277958643984933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2840.1577795308836765No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA2740.15222391359903997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG2690.14944610495672173No Hit
GGCCAAAAGTGTACGGAGGGAGATTATCATAGTGTTGACAGTAGTATGTTGCAATATCTTCAGGCTGCAGGCTG2680.1488905432282581No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT2680.1488905432282581No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC2680.1488905432282581No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2630.14611273458593985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG2610.14500161112901255No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT2460.1366681852020578No Hit
ACATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCA2410.13389037655973957No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC2390.13277925310281224No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2360.1311125679174213No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC2300.1277791975466394No Hit
ATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT2210.12277914199046655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2210.12277914199046655No Hit
GTAGTATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACGTCCA2200.12222358026200292No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2190.12166801853353926No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2120.11777908643429372No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT2090.11611240124890278No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA2040.11333459260658452No Hit
ATACTACTGTCAACACTATGATAATCTCCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA2040.11333459260658452No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2010.11166790742119356No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC1990.11055678396426627No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT1990.11055678396426627No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA1940.10777897532194802No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG1930.10722341359348438No Hit
CCCCTAAACTCCTGATCTACGATGCATCCATTTTGGAAACAGGGGTCCCA1900.10555672840809342No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC1860.10333448149423882No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT1850.10277891976577518No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC1840.10222335803731153No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTACGATGCATCCATTTTGGAAACAGGGGTCCCATCAAGGTT1830.10166779630884787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCA302.4244506E-455.81052469
ACAATAT350.00624810753.89945270
GCGCCAC200.00703290852.32827852
CGAAGCG200.007442070651.5855123
GTGGTAT901.2551027E-1038.2327731
GTATCAA4650.036.259471
TATCAAC5250.031.4425982
TCAACGC5550.029.07484
TGGGGAT500.006915663428.22718664
ATCAACG5950.027.7049163
TTATTTA500.00790278527.4664069
AACGCAG6150.026.7964956
CAACGCA6050.026.6719255
ACGCAGA6150.026.2382347
AGAGTAC6500.025.36770211
GGGACAA700.00127712624.81314345
CGCAGAG6700.024.0843498
CAGAGTA6750.023.9125910
CAGTTCG3202.419256E-1023.58101370
GCAGAGT6900.023.3862539
Produced by FastQC (version 0.11.5)