FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955503
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48400.5065394875787936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34360.35960117341337494No Hit
GTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGT32350.338565132710206No Hit
GATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA31250.3270528716288698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30330.3174244350881159No Hit
GAGTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT29740.31124967687176286No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29720.31104036303392035No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29120.30476094789864605No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28770.30109795573640274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26830.28079451346568246No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26510.27744549206020286No Hit
GTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGA26450.27681755054667545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26310.2753523536817781No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24780.2593398450868286No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24300.2543163129786092No Hit
ACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCACTTTCGGCGG23290.24374596416756408No Hit
GGAGGGGACTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGT22850.2391410597350296No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAA22480.2352687537349438No Hit
GACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCC22360.23401287070788893No Hit
GTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA22360.23401287070788893No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22010.23034987854564562No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21760.22773345557261462No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTC21590.22595428795095357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21570.22574497411311112No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20990.21967487281567927No Hit
CCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT20800.21768639135617576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20480.2143373699506961No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19870.20795329789650058No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19640.20554618876131212No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA18660.19528981070703075No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT18250.19099887703125998No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATT18150.18995230784204759No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18150.18995230784204759No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17900.18733588486901664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17550.18367289270677328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16710.17488171151738927No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16460.17226528854435832No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.17017215016593354No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAACTCAACTTCAGGGAGA15950.16692778567937516No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15920.16661381492261146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15910.1665091580036902No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA15850.16588121649016277No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15790.16525327497663533No Hit
GTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT15760.16493930421987163No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14850.15541552459803895No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14790.15478758308451152No Hit
TCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT14680.1536363569763779No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14580.1525897877871655No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGA14340.1500780217330558No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14320.1498687078952133No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14300.14965939405737083No Hit
CACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCA13960.14610105881404872No Hit
GTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATTGGAGTTGGAT13950.14599640189512747No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13870.14515914654375758No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13620.14254272357072661No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13440.14065889903014434No Hit
CTCCTGATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGA13400.14024027135445938No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12910.1351120823273187No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12820.13417017005702755No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12520.1310304624893904No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12350.12925129486772935No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12060.12621624421901345No Hit
GGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCC12000.12558830270548602No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA12000.12558830270548602No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11990.1254836457865648No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAG11920.1247510473541161No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11900.12454173351627362No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11750.12297187973245505No Hit
GACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCT11710.1225532520567701No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11670.12213462438108513No Hit
GTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAACCAGGTAAAGCCCCT11500.1203554567594241No Hit
GAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGGCTCCTGATCTATGGAGCATC11480.12014614292158161No Hit
GTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTA11450.1198321721648179No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11430.11962285832697542No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11310.11836697529992056No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11250.11773903378639314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCC11160.116797121516102No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11050.11564589540796837No Hit
GATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATTTAAAA10990.11501795389444094No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCTCCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTG10910.11418069854307103No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10770.1127155016781737No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT10710.11208756016464626No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10620.11114564789435513No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10400.10884319567808788No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10220.10695937113750559No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10180.10654074346182064No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10100.10570348811045072No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10090.10559883119152949No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10070.10538951735368703No Hit
GGTAAAGCCCCTAGGCTCCTGATCTATGGAGCATCCAGTTTGCAAAGTGG10030.10497088967800206No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10000.10465691892123834No Hit
GCAGAAACCAGGTAAAGCCCCTAGGCTCCTGATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT9990.1045522620023171No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9910.10371500665094718No Hit
CCCCTAGGCTCCTGATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCA9890.10350569281310472No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTG9880.10340103589418348No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9730.1018311821103649No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9730.1018311821103649No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAGGGGACTGTAACTCTGTTG9600.10047064216438882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)