FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955503
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51510.5390877893632987No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31640.3311344914667981No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30690.32119208416928047No Hit
GTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGT30460.318784975034092No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29770.3115636476285265No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29120.30476094789864605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28590.2992141311958204No Hit
GATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCA28580.29910947427689916No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28140.2945045698443647No Hit
GAGTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT27710.29000432233075146No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTCCGCCGAAAGTG26640.2788060320061789No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25510.26697980016807904No Hit
GTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGA24280.2541069991407667No Hit
GGAGGGGACTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGT23910.25023469314068086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23740.24845552551901984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23020.24092022735669066No Hit
GACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCC22220.2325476738429916No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22100.23129179081593673No Hit
GTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA21490.2249077187617412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21290.22281458038331645No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAA20830.21800036211293947No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCTAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTC20600.21559325297775098No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20510.21465134070745984No Hit
ACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCACTTTCGGCGG20310.21255820232903505No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20180.21119766238305898No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19980.2091045240046342No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19860.20784864097757935No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19510.20418564881533602No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19300.20198785351799No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT19260.20156922584230508No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA19070.19958074438280152No Hit
CCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT18780.1965456937340856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17200.18000990054452995No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17200.18000990054452995No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17020.17812607600394767No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16920.17707950681473528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16840.17624225146336536No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCA16800.17582362378768043No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATT16560.1733118577335707No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16030.16776504103074508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15950.16692778567937516No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15410.16127631205762827No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15190.15897385984136103No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14980.15677606454401505No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14870.15562483843588143No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAACTCAACTTCAGGGAGA14640.15321772930069294No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGTCAAGTCAGA14450.1512292478411894No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14400.15070596324658322No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14350.15018267865197701No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14320.1498687078952133No Hit
GTTACAGTCCCCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT14140.147984883354631No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13620.14254272357072661No Hit
CTCCTGATCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGA13480.14107752670582926No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13440.14065889903014434No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13420.14044958519230186No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13400.14024027135445938No Hit
TCTATGGAGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT13180.13793781913819214No Hit
GTCTCTGGTGGCTCCATCAGCAATAATGGTGATTACTATTGGAGTTGGAT13150.1376238483814284No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13140.13751919146250718No Hit
GGTTGTAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCC13050.13657727919221604No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCC13010.1361586515165311No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACT12810.13406551313810633No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12780.1337515423813426No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12590.13176306092183906No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12150.1271581564893046No Hit
CACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTACTACTGTCAACAGAGTTACAGTCCCCTCCCGCTCA11820.12370447816490372No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11630.1217159967054002No Hit
GACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCT11630.1217159967054002No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA11590.12129736902971523No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11520.12056477059726657No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11350.11878560297560552No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11320.1184716322188418No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11300.11826231838099933No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11250.11773903378639314No Hit
GAGTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAACCAGGTAAAGCCCCTAGGCTCCTGATCTATGGAGCATC11120.11637849384041703No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10930.11439001238091351No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAG10480.1096804510294578No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10400.10884319567808788No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10400.10884319567808788No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10400.10884319567808788No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10380.10863388184024539No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCTCCATAGATCAGGAGCCTAGGGGCTTTACCTGGTTTCTG10370.10852922492132416No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10360.10842456800240292No Hit
GTTCAGTGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTAGCAACTTA10350.10831991108348167No Hit
GTATAAAGAGGTTTTTAAATTGGTATCAGCAGAAACCAGGTAAAGCCCCT10330.1081105972456392No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10260.10737799881319054No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCACTGAACCTTG9970.10434294816447462No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9860.103191722056341No Hit
GATCAGGAGCCTAGGGGCTTTACCTGGTTTCTGCTGATACCAATTTAAAA9850.10308706513741978No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9840.10298240821849854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9730.1018311821103649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.051.2958681
GTATCAA18800.040.4870951
TACTGAA5800.040.4085670
CTCGAGA400.00263941534.3838212
TATCAAC22200.033.7979052
ATCAACG22200.033.7678263
TCAACGC22400.033.454074
CAACGCA22950.032.5025565
AACGCAG23300.032.161856
TCCGATG550.004655398430.62127769
CGCAGAG25300.029.4835458
GTAGTTT2152.062734E-929.46193370
TCTACTG4900.029.05922368
AGAGTAC26300.028.49767511
GTCTACT4800.028.47089667
TGTTAGA8850.027.91393370
GCAGAGT27250.027.6260059
CGTCGGC500.007709183327.61837436
TTAGGTG500.00787267727.499864
ACGCAGA27300.027.4494937
Produced by FastQC (version 0.11.5)