FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533951
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20870.13605388959621267No Hit
GTCTCTGGTGGCTCCATCATCAGTGGTGATAACTACTGGAGTTGGATCCG18280.11916938676659163No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16000.10430580898607583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28250.039.7045631
TATCAAC33150.033.930972
ATCAACG33900.033.17923
TCAACGC34200.032.788344
CAACGCA34650.032.362525
AACGCAG35200.031.9548766
AGAGTAC38650.029.00755311
ACGCAGA40550.027.8194337
GTGGTAT6150.027.4977041
CGCAGAG40800.027.4798648
GGTATCA18800.023.6813961
AGTACGG38100.023.2694113
GAGTACG38350.023.20767212
ATACCCG750.00199217523.0022835
GCAGAGT49500.022.7197639
CAGAGTA49900.022.46850610
CGAACTA1756.170012E-921.69635429
TACGGGA17450.020.36193515
GTACGGG45600.019.36655414
GAGTACT12600.018.61725412
Produced by FastQC (version 0.11.5)