FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533951
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18310.11936496015844053No Hit
GTCTCTGGTGGCTCCATCATCAGTGGTGATAACTACTGGAGTTGGATCCG18180.11851747546042866No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17080.11134645109263594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5050.037.5901221
GTATCAA28950.036.6009371
TATCAAC33500.031.6111832
ATCAACG34200.030.8401583
TCAACGC34600.030.4687164
CAACGCA34750.030.3352225
AACGCAG36250.028.9849386
GGTATCA19750.027.786411
AGAGTAC38900.026.7490711
CATAACG950.00916445726.65602570
CGCAGAG39650.026.5863538
ACGCAGA40500.026.028377
TCGCGAC700.001273071324.84148650
CCGGAGT11600.024.55916270
AGTACGG36500.022.56531113
GAGTACG37550.021.93289412
GCAGAGT50100.021.1089699
CAGAGTA50250.020.84097710
GTAGTTT4503.8198777E-1119.69584370
ATTTCGT900.005768966419.14724216
Produced by FastQC (version 0.11.5)