FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134186
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43580.38424032742424963No Hit
ATGTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35040.3089440356343669No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA34360.30294854635835744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28720.2532212529514559No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28080.2475784395152118No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT26390.2326778852851296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25200.22218577905211315No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23790.20975395570038777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23590.20799057650156147No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23230.20481649394367413No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT22950.20234776306531735No Hit
GTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGA22110.1949415704302469No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21160.18656551923582199No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20560.1812753816393431No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20470.18048186099987126No Hit
GTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA20170.17783679220163182No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19800.17457454068380318No Hit
CTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19760.17422186484403793No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC19570.17254665460515295No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19230.16954890996714825No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18760.16540496884990646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18390.1621427173320778No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18240.16082018293295808No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18210.16055567605313414No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17250.15209145589876794No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17050.15032807669994164No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA16750.1476830079017022No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16450.14503793910346274No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16220.1430100530248125No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16210.1429218840648712No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16140.142304701345282No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15870.1399241394268665No Hit
GTATAAAACACTCTGGGAGGACTTGCAGTTGATGGTGGCCCTCGCGCCCAGAGACACAGACAGGGAGGCTGGAGA15630.13780808438827494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15530.1369263947888618No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGAGTGTTTTATA15460.1363092120692726No Hit
CACCTGCAGTGTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGA14990.1321652709520308No Hit
GTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGAGTTGGATCCG14980.1320771019920895No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14620.12890301943420215No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14450.1274041471151998No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14260.1257289368763148No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13700.12079147511960119No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13560.1195571096804228No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13180.11620668920265281No Hit
GGACTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13150.11594218232282888No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGCCTCCCTGTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTG13070.11523683064329836No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12870.11347345144447206No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12780.11267993080500023No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12550.11065204472635No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTATTGTCAGCAATGTTATAGTCCT12450.10977035512693685No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12320.10862415864769975No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12310.10853598968775845No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12090.10659627256904952No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12080.1065081036091082No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12050.10624359672928427No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12030.10606725880940163No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11890.10483289337022324No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11880.1047447244102819No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTATTGTCAGCAATGTTATAGTCCTCCGTGGACGTT11860.10456838649039928No Hit
GTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGG11780.10386303481086877No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11620.10245233145180772No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11580.10209965561204246No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11570.10201148665210115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11550.10183514873221852No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11510.10148247289245325No Hit
GGTATGGAGATTACCACTGGAGTTGGATCCGCCAGCCCCCAGGAAAGGGC11490.10130613497257063No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11380.10033627641321617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)