FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005760883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134186
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42050.37075047655322846No Hit
ATGTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT35740.3151158628302589No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA31600.2786139134145546No Hit
CTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28050.24731393263538784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26430.23303056112489484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26380.2325897163251883No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25030.22068690673311078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23880.2105474763398596No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23390.2062271973027352No Hit
CTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT22820.20120156658608024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22230.19599959794954266No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21630.19070946035306377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21470.18929875699400275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20580.18145171955922573No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT20130.1774841163618666No Hit
GTTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA19800.17457454068380318No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19270.1699015858069135No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18110.159673986453721No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18080.15940947957389706No Hit
GTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGA17530.15456018677712474No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17400.15341399029788766No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17350.15297314549818108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17220.15182694901894397No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17160.15129793525929608No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17110.15085709045958953No Hit
GGACTATAACATTGCTGACAATAATAAATTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16880.14882920438093927No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16160.14248103926516462No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15950.14062949110639703No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15760.13895428086751202No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTCCTGGGCATCTACCCGGGAA15160.13366414327103315No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14850.13093090551285239No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT14720.1297847090336153No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14640.12907935735408477No Hit
GTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGAGTTGGATCCG14640.12907935735408477No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14630.12899118839414347No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14370.12669879543566928No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14160.12484724727690166No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14100.12431823351725377No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13740.12114415095936645No Hit
CACCTGCAGTGTCCCTGGTGGCTCCATCAGGTATGGAGATTACCACTGGA13540.11938077176054016No Hit
GTCTCCAGCCTCCCTGTCTGTGTCTCTGGGCGCGAGGGCCACCATCAACTGCAAGTCCTCCCAGAGTGTTTTATA13100.11550133752312232No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13000.11461964792370916No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12840.11320894456464813No Hit
GTATAAAACACTCTGGGAGGACTTGCAGTTGATGGTGGCCCTCGCGCCCAGAGACACAGACAGGGAGGCTGGAGA12750.1124154239251763No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12490.11012303096670212No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12370.10906500344740634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12370.10906500344740634No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12340.10880049656758238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12340.10880049656758238No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12330.10871232760764109No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12330.10871232760764109No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12280.1082714828079345No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTATTGTCAGCAATGTTATAGTCCT12070.1064199346491669No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT11750.10359852793104482No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11670.10289317625151431No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11660.10280500729157299No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11440.10086529017286407No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTATTGTCAGCAATGTTATAGTCCTCCGTGGACGTT11350.10007176953339222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)