FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292999
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8520.29078597537875556No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC3220.10989798599995222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC200.00775843851.053227
TAGGGCG350.001400647539.1115836
GTATCAA7650.036.0498731
GTGGTAT1850.034.9672971
ACCGGGG400.002790037533.988998
TGGTATC1900.032.22762
ATCAACG8750.030.7029253
GCTTATA450.004925383730.264091
AACGCAG9100.030.2539396
CAACGCA9100.029.8804325
TCAACGC9000.029.834784
GGTATCA3600.028.3725831
CGATTCG605.9062376E-428.324169
TACGATT605.9062376E-428.324167
CGCAGAG9750.028.2370078
ACGCAGA9900.027.8091757
AGAGTAC10100.027.25849711
CCTACAT500.00828837927.2051243
TTATACT500.00830911827.1911934
TATCAAC9900.027.1456952
Produced by FastQC (version 0.11.5)