FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742837
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTGTATAAAACACT27880.3753178691960686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25750.3466440147704005No Hit
CTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT24790.33372058742362054No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23860.3212010171814274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20160.2713919742823796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20050.2699111648988944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19170.2580646898310127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16950.22817926409158404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16940.22804464505672173No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16680.22454455015030214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16600.22346759787140383No Hit
GTTTTATACAGCTCCACCAATAAGAATTATTTAGCTTGGTACCAGCAGAA16040.2159289319191155No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15860.21350578929159428No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA15320.20623636140903054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14900.2005823619448143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14510.1953322195851849No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCAC14370.19344755309711284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13970.18806279170262116No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCCA13800.18577426810996223No Hit
ATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13700.1844280777613393No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13570.1826780303081295No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12620.16988922199621181No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12260.16504293674116932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12180.163965984462271No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12080.16261979411364808No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11820.15911969920722852No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC11780.15858122306777933No Hit
ATTCAGTGGCAGCGGGTTTGGGACAGATTTCACTCTCACCATCAGCAGCC11530.15521574719622205No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11210.15090793808062872No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11040.14861941448796978No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10860.1461962718604485No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10680.1437731292329273No Hit
GTTTATTACTGTCAGCAATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10430.14040765336136998No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCCACTGAATCGGTCAGGGACC10170.1369075584549504No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10130.13636908231550127No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTATT9940.1338113206531177No Hit
GTCCCAAACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT9930.13367670161825543No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9910.13340746354853084No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAATAATTCTTATTGGTGGAGCTG9720.1308497018861473No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9580.12896503539807522No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9560.12869579732835063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9490.1277534640843146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9420.12681113084027854No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG9160.12331103593385899No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9100.12250332172468521No Hit
GAGTGAAATCTGTCCCAAACCCGCTGCCACTGAATCGGTCAGGGACCCCG8990.12102251234120001No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCTTCTACCCGGGAA8970.12075327427147543No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA8670.11671470322560669No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8670.11671470322560669No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8660.11658008419074439No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT8590.11563775094670836No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC8580.11550313191184608No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8460.11388770349349858No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8160.10984913244762984No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8090.1089067992035938No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCCACTGAATCGGT8040.10823370402928234No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAAACCCGCTGCCA7970.10729137078524628No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTATTCCTCAGACTTTTGGCCAGGGGACCAAG7850.10567594236689878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830.10540670429717422No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT7720.103925894913689No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7670.10325279973937755No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7490.10082965711185629No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7480.10069503807699401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC200.006327390753.76264667
GTGGTAT2300.046.425891
GGGCGTA306.3894276E-445.883383
GGTATCA7350.042.646211
TGGTATC2750.040.0598682
GTATAGA451.01066966E-438.272241
GTATCAA15950.037.792341
TATCAAC17850.033.5585562
ATCAACG18550.032.2791443
TCAACGC18700.032.0008244
TATTCTA1202.333245E-531.815270
CAACGCA18800.031.4626165
AACGCAG19150.030.887586
CGATTGT850.005190754329.94371870
TACGAAT605.3556403E-428.80906938
CGCAGAG20950.028.2337558
TGTTAGA7000.028.17917870
AGAGTAC21400.027.8063711
ACGCAGA21350.027.7047867
CAGAGTA21500.027.6733110
Produced by FastQC (version 0.11.5)