FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953517
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51210.262142586934232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35750.18300327051159526No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG33090.16938680339101223No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31470.16109406777622104No Hit
GTATGATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT30630.15679413079077378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29480.15090731229879237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29240.14967875887437887No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29200.14947399997030994No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28840.14763116983368968No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27940.14302409449213904No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAA27760.14210267942382893No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26490.13560158421964078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24450.12515888011212598No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT24290.12433984449585031No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGCTCACTTTCGGCGGAGGGA23960.12265058353728173No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23160.11855540545590339No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC22630.11584234997699021No Hit
GTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA21540.11026266984111222No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGTACA21360.10934125477280207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21120.10811270134838857No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20640.10565559449956156No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20070.10273778011657948No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19930.10202112395233827No Hit
CAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA19800.1013556575141143No Hit
TTATCATACTGTTGACAGTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATC19770.10120208833606259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57550.039.9661641
TCGACGT2350.039.54955729
CTCGACG2400.038.71965428
CGACGTC2500.037.1813530
CGTATGC1700.036.6723141
GTGGTAT6700.035.9198841
ACTCGAC2600.035.73114427
CGACCGG1554.125468E-935.320970
TATCAAC65600.035.1593972
TCAACGC66450.034.657044
ATCAACG66350.034.6565783
CAACGCA67700.033.9663665
CTCGTAT1550.033.46418839
AACGCAG69200.033.2301036
GACTCGA2750.032.52431526
ACGAGAC2800.031.92634822
TTAACGG2400.031.61985435
CGCGGTA2550.031.28213943
TCGTATG1900.030.96281840
ACGCAGA75250.030.5112047
Produced by FastQC (version 0.11.5)