FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953517
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51110.2616306896740596No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG34660.17742359037571723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30320.15520724928423965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29950.15331322942160217No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29630.1516751581890508No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29260.14978113832641332No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28970.1482966362719137No Hit
GTATGATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAACTGTGGCTGCACCAT28960.14824544654589647No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGCAACTATTTAACTTGGTATCAGAAGAAA28000.14333123284824242No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27220.13933843421889855No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26220.1342194616171756No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25560.13084093970003843No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23020.11783874929166217No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG22780.11661019586724866No Hit
GATCTACGCTGCATCCGATTTGGAGGCAGGGGTCCCATCACGGTTCAGTGGAAGTGGATCTGGGATACATTTTA22720.11630305751114528No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT21640.1107745671012845No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21370.1093924444988193No Hit
ATATTACTGTCAACAGTATGATAATCTCCCGCTCACTTTCGGCGGAGGGA21320.10913649586873315No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20770.10632106093778554No Hit
GTAATATATTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTATCCCAGATCCACTTCCA20250.1036591951848896No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC20140.10309610819870009No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20020.10248183148649334No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19670.1006901910758903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7900.042.2213331
GTATCAA57800.039.9787061
TATCAAC64900.035.6324772
TCAACGC68500.033.878194
ATCAACG68700.033.794273
CAACGCA69600.033.3427625
AACGCAG70500.032.819726
CGCAGAG76200.030.36478
ACGCAGA76550.030.225877
AGAGTAC77100.029.8789711
CAGAGTA78400.029.42505610
GCAGAGT80200.028.7218429
AGTACGG59500.025.7981213
GAGTACG59600.025.69393212
GAGTACT32800.023.44855712
GGTATCA43600.023.3449271
CGTCTAA1253.178735E-622.3302350
GTACGGG69950.021.89554814
CCGTTAA2209.094947E-1221.85426716
ACGGGGG13050.021.84230416
Produced by FastQC (version 0.11.5)