FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590225
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGATACAACTATTTGGATTGGTACCTGCAAAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT16200.2744715998136304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16070.2722690499385827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10680.18094794358083782No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10140.1717988902537168No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTC9700.16434410606124783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9600.1626498369265958No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8950.15163708755135752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8940.15146766063789233No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8420.14265746113770172No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7920.13418611546444154No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT7770.13164471176246348No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7710.13062815028167224No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA7600.128764454233555No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA7100.1202931085602948No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG6510.11029692066584777No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6420.10877207844466094No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC6390.10826379770426532No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTATTGGATACAACTATTTGGATTGGTACCTGCAAAAGCCAGGGCAGTCTC6350.10758609005040451No Hit
ATCCAATACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC6280.10640010165614809No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5950.10080901351179636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA350.001266767239.92582756
CGTATGC350.00132221839.57947541
CTAGATC350.001339446639.47539533
GGTATCA6350.038.0411641
TGGTATC1950.037.1538472
GTGGTAT2100.036.1520461
GTATCAA12700.036.1391071
ATATAGC652.3196211E-531.8461533
TATCAAC14850.030.8989892
TCAACGC15100.030.8443684
TCGTATG450.004546662330.76833340
CTCGTAT450.004554231730.75787539
ACTAGAT450.004601764530.6926732
ATCAACG15250.030.5409833
CAACGCA15500.029.8258045
AACGCAG16000.028.8937516
CGAACGT605.3628563E-428.80124536
ACGGGTA605.421731E-428.74756616
AGAGTAC16700.028.09342811
ACGCAGA16500.028.0158087
Produced by FastQC (version 0.11.5)