FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026249
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.040.5729521
GTATCAA60650.040.2971
ATCAACG72400.033.9411353
TCAACGC73250.033.4542664
TATCAAC73750.033.3206672
CAACGCA74250.033.0037045
AACGCAG76850.031.9765666
ACGCAGA82150.029.8276757
AGAGTAC82900.029.30619811
CGCAGAG83950.029.1881338
GTGGTAT9100.028.7082121
CAGAGTA85700.028.26920710
GCAGAGT88700.027.6617689
TGGTATC9600.025.776912
AGTACGG66350.024.96322413
GAGTACG66450.024.92565712
TCGTATG2155.456968E-1222.55138640
GTACGGG77600.021.29991714
TACGGGA25650.020.76584615
AGTACTT36800.020.63664213
Produced by FastQC (version 0.11.5)