FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005760897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005760897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026249
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25960.1281185086334404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67000.038.3996431
GGTATCA28200.036.9567641
ATCAACG78300.032.5818863
TATCAAC78550.032.5508352
TCAACGC78450.032.5051424
GTGGTAT12200.031.858041
CAACGCA80000.031.8308855
AACGCAG82750.031.0633726
ACGCAGA87000.029.3881177
TGGTATC12800.029.2652262
AGAGTAC87550.029.04885511
CGCAGAG89300.028.6689248
CAGAGTA89600.028.4580310
GCAGAGT92350.027.7964139
ACGGGTA5700.025.29955316
GAGTACG73650.024.28205912
AGTACGG74050.024.05996513
AGTACTT34750.021.43668413
GAGTACT35000.021.38006412
GTACGGG84150.021.00953914
Produced by FastQC (version 0.11.5)